ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

 

  • This study was conducted to develop a self-assembled direct competitive enzyme-linked immunosorbent assay (dcELISA) kit for the detection of deoxynivalenol (DON) in food and feed grains.

 

    • Based on the preparation of anti-DON monoclonal antibodies, we established a standard curve with dcELISA and optimized the detection conditions.

 

 

    • The performance of the kit was evaluated by comparison with high-performance liquid chromatography (HPLC). The minimum detection limit of DON with the kit was 0.62 ng/mL, the linear range was from 1.0 to 113.24 ng/mL and the half-maximal inhibition concentration (IC50) was 6.61 ng/mL in the working buffer; there was a limit of detection (LOD) of 62 ng/g, and the detection range was from 100 to 11324 ng/g in authentic agricultural samples.

 

    • We examined four samples of wheat bran, wheat flour, corn flour and corn for DON recovery. The average recovery was in the range of 77.1% to 107.0%, and the relative standard deviation (RSD) ranged from 4.2% to 11.9%. In addition, the kit has the advantages of high specificity, good stability, a long effective life and negligible sample matrix interference. Finally, wheat samples from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China were analyzed by the kit.

      iotanano
      iotanano

 

    • A total of 30 samples were randomly checked (five samples in each province), and the results were in good agreement with the standardized HPLC method.

 

  • These tests showed that the dcELISA kit had good performance and met relevant technical requirements, and it had the characteristics of accuracy, reliability, convenience and high-throughput screening for DON detection. Therefore, the developed kit is suitable for rapid screening of DON in marketed products.

 

BD-1 Beta Defensin-1 Mouse Recombinant Protein

PROTP56386 Regular: 20ug
EUR 380.4
Description: BD 1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 37 amino acids and having a molecular mass of 4.1 KDa.;The BD 1 Mouse is purified by proprietary chromatographic techniques.

BD-1 Beta Defensin -1 Rat Recombinant Protein

PROTO89117 Regular: 20ug
EUR 380.4
Description: BD-1 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 37 amino acids and having a molecular mass of 4.1kDa.;The BD-1 is purified by proprietary chromatographic techniques.

Human Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit

EI2200-1 96 Well Plate
EUR 572.4

Recombinant Human BD-1 (36 a.a.) Protein

PROTP60022-1 20ug
EUR 380.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-1 is a 3.9 kDa protein containing 36 amino acid residues.

Human BD-4/beta-Defensin 4 ELISA Kit

RK00186 96 Tests
EUR 625.2

Human BD-2/beta-Defensin 2 ELISA Kit

RK00193 96 Tests
EUR 625.2

BD-2, beta-Defensin 2, human

RC220-13 5ug
EUR 125.26

BD-3, beta-Defensin 3, human

RC220-14 5ug
EUR 125.26

BD-4, beta-Defensin 4, human

RC220-15 5ug
EUR 125.26

Mouse Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit

EMI2200-1 96 Well Plate
EUR 572.4

Human TGF-beta-1 AssayMax ELISA Kit

ET3102-1 96 Well Plate
EUR 572.4

Human beta-Defensin 1 (BD-1/hBD-1) control/blocking peptide #1

HBD12-P 100 ug
EUR 196.8

Rabbit Anti-Human beta-Defensin 1 (BD-1/hBD-1) antiserum #1

HBD12-S 100 ul
EUR 548.4

Nori® Human b-defensin 1 (BD-1) ELISA Kit

GR111105 96-well
EUR 553.2

Bovine Beta-defensin 1(DEFB1) ELISA kit

1-CSB-E17795B
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Beta-defensin 1(DEFB1) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Monkey Beta-defensin 1(DEFB1) ELISA kit

1-CSB-EL006662RH
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Monkey Beta-defensin 1(DEFB1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Human Brain Genomic DNA  

X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

Human Defensin Beta 2 (BD-2) Protein

abx670204-20ug 20 ug
EUR 661.2

Human beta-Defensin 3 (BD-3) control/blocking peptide #1

HBD31-P 100 ug
EUR 196.8

Defensin Beta 2 (BD-2) Antibody

20-abx148604
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Defensin Beta 2 (BD-2) Antibody

20-abx007740
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Defensin Beta 2 (BD-2) Antibody

abx412569-01mg 0.1 mg
EUR 844.8

Recombinant Human BD-4 Protein

PROTQ8WTQ1-1 20ug
EUR 380.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces.  In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells.  The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.   BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.  In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes.  Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Recombinant Human BD-3 Protein

PROTP81534-1 20ug
EUR 380.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.

Nori® Equine b-defensin 1 (BD-1) ELISA Kit

GR106586 96-well
EUR 553.2

Nori® Porcine b-defensin 1 (BD-1) ELISA Kit

GR113087 96-well
EUR 553.2

Nori® Canine b-defensin 1 (BD-1) ELISA Kit

GR115106 96-well
EUR 553.2

Mouse Beta-defensin 1 (Defb1)

1-CSB-EP006662MO
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Beta-defensin 1(Defb1) expressed in E.coli

Rabbit Anti-Human beta-Defensin 1 (BD-1/hBD-1), IgG #1, aff pure

HBD12-A 100 ug
EUR 578.4

Human beta Defensin-1

90106-A 5 µg
EUR 130
Description: Recombinant beta Defensin-1 is a disulfide-linked monomer protein consisting of 47 amino acid residues, and migrates as an approximately 5 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human beta Defensin-1 mature chain was expressed in E. coli.

Human beta Defensin-1

90106-B 20 µg
EUR 205
Description: Recombinant beta Defensin-1 is a disulfide-linked monomer protein consisting of 47 amino acid residues, and migrates as an approximately 5 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human beta Defensin-1 mature chain was expressed in E. coli.

 

 

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.

Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages.

 

Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F.

 

hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees.

 

Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied.Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds.

 

In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples.

 

Nori® Porcine b-defensin 1 (BD-1) ELISA Kit- 2 Plates

GR113087-2 2 x 96-well
EUR 998.4

BD-2, beta-Defensin 2, human

RC220-13 5ug
EUR 125.26

Human BD-2/beta-Defensin 2 ELISA Kit

RK00193 96 Tests
EUR 625.2

Recombinant Human BD-2 Protein

PROTO15263-2 20ug
EUR 380.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-2 is a 4.3 kDa protein containing 41 amino acid residues.

Defensin Beta 2 (BD-2) Antibody

20-abx148604
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Defensin Beta 2 (BD-2) Antibody

20-abx007740
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Defensin Beta 2 (BD-2) Antibody

abx412569-01mg 0.1 mg
EUR 844.8

Human Defensin Beta 2 (BD-2) Protein

abx670204-20ug 20 ug
EUR 661.2

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag

E80025
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag

E80027
  • EUR 635.80
  • EUR 4087.60
  • 1 ml
  • 100 ul

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial

E80028
  • EUR 388.30
  • EUR 860.20
  • 20 ul
  • 100 ul

SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag

E80024
  • EUR 635.80
  • EUR 4995.10
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion

E80026
  • EUR 588.50
  • EUR 823.90
  • 20 ul
  • 50 ul

SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag

E80021
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag

E80023
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag

E80020
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag

E80022
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

Defensin Beta 2 (BD-2) Antibody (Biotin)

abx412570-50ug 50 ug
EUR 861.6

Nori® Porcine b-defensin 2 ELISA Kit- 2 Plates

GR113081-2 2 x 96-well
EUR 998.4

BD-2 Beta Defensin-2 Human Recombinant Protein

PROTO15263-1 Regular: 20ug
EUR 380.4
Description: Beta Defensin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.3 kDa. ;The BD-2 is purified by proprietary chromatographic techniques.

Human beta-Defensin-2 (BD-2) ELISA Kit, 96 tests, Quantitative

100-250-BD2 1 Kit
EUR 1074

Recombinant Transmembrane Protease Serine 2 Protein, Partial

E80016
  • EUR 470.80
  • EUR 753.50
  • EUR 1824.90
  • 20 ul
  • 100 ul
  • 500 ul

BD-2 Beta Defensin-2 Mouse Recombinant Protein

PROTO15263 Regular: 20ug
EUR 380.4
Description: Beta Defensin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.5kDa. ;The BD-2 is purified by proprietary chromatographic techniques.

Nori Human LH beta ELISA Kit-2 Plates

GR111054-2 2 x 96-well
EUR 998.4

Nori Porcine LH beta ELISA Kit-2 Plates

GR113014-2 2 x 96-well
EUR 998.4

Nori® Human NRG1-beta 1/HRG1-beta 1 ELISA Kit (2 plates)

GR106283-2 2 x 96-well
EUR 998.4

Nori® Human TGF beta 1 ELISA Kit-2 Plates

GR106058-2 2 x 96-well
EUR 998.4

Nori® Human IL-1 beta ELISA Kit (2 plates)

GR106393-2 2 x 96-well
EUR 998.4

Nori® Human PKA RI-beta ELISA Kit- 2 Plates

GR111083-2 2 x 96-well
EUR 998.4

Nori® Human b-defensin 2 (BD-2) ELISA Kit

GR111099 96-well
EUR 553.2

Human beta-Defensin 2 (BD-2/hBD-2)control/blocking peptide #1

HBD21-P 100 ug
EUR 196.8

Rabbit Anti-Human beta-Defensin 2 (BD-2/hBD-2)antiserum #1

HBD21-S 100 ul
EUR 548.4

ACE2, His-Tag Protein

E80019
  • EUR 612.70
  • EUR 1437.70
  • 20 ul
  • 100 ul

Human BD-4/beta-Defensin 4 ELISA Kit

RK00186 96 Tests
EUR 625.2

Human BD-1/beta-Defensin 1 ELISA Kit

RK00211 96 Tests
EUR 625.2

Nori Human TIM-2 ELISA Kit-2 Plates

GR111039-2 2 x 96-well
EUR 998.4

Nori® Human Angiopoietin-2 (ANG-2) ELISA Kit (2 plates)

GR106245-2 2 x 96-well
EUR 998.4

Nori® Equine PKA RI-beta ELISA Kit- 2 Plates

GR106567-2 2 x 96-well
EUR 998.4

Nori® Bovine PKA RI-beta ELISA Kit- 2 Plates

GR112036-2 2 x 96-well
EUR 998.4

Nori® Porcine PKA RI-beta ELISA Kit- 2 Plates

GR113069-2 2 x 96-well
EUR 998.4

Nori® Chicken PKA RI-beta ELISA Kit- 2 Plates

GR114016-2 2 x 96-well
EUR 998.4

Nori® Human Tie-2 ELISA Kit (2 plates)

GR106458-2 2 x 96-well
EUR 998.4

Nori® Human TIMP-2 ELISA Kit (2 plates)

GR106460-2 2 x 96-well
EUR 998.4

Nori® Human Trappin-2 ELISA Kit (2 plates)

GR106467-2 2 x 96-well
EUR 998.4

BD-3, beta-Defensin 3, human

RC220-14 5ug
EUR 125.26

BD-4, beta-Defensin 4, human

RC220-15 5ug
EUR 125.26

Nori® Human MMP-2/TIMP-2 Complex ELISA Kit (2 plates)

GR106372-2 2 x 96-well
EUR 998.4

Human beta defensin 2 ELISA Kit

ELA-E0377h 96 Tests
EUR 988.8

Recombinant Human BD-1 (47a.a.) Protein

PROTP60022-2 20ug
EUR 380.4
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

Nori® Equine (horse) TGF-beta 1 ELISA Kit (2 plates)

GR106123-2 2 x 96-well
EUR 998.4

Nori® Bovine (cattle) TGF-beta 1 ELISA Kit (2 plates)

GR106124-2 2 x 96-well
EUR 998.4

Nori® Canine (dog) TGF-beta 1 ELISA Kit (2 plates)

GR106125-2 2 x 96-well
EUR 998.4

Nori® Porcine (pig) TGF-beta 1 ELISA Kit (2 plates)

GR106126-2 2 x 96-well
EUR 998.4

Recombinant Human TGF-Beta 2 (insect derived) Protein

PROTP61812-2 10ug
EUR 380.4
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-β2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
* Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

Anti-beta 2 Microglobulin/B2m Antibody

A00456-2 100ug/vial
EUR 352.8

Nori Equine MMP-2 ELISA Kit-2 Plates

GR106478-2 2 x 96-well
EUR 998.4

Nori Equine TIM-2 ELISA Kit (2 plates)

GR106516-2 2 x 96-well
EUR 998.4

Nori Canine TIM-2 ELISA Kit (2 plates)

GR115041-2 2 x 96-well
EUR 998.4

Nori Equine MMP-2/TIMP-2 Complex ELISA Kit (2 plates)

GR106502-2 2 x 96-well
EUR 998.4

Human beta Defensin-2

90107-A 5 µg
EUR 130
Description: Recombinant beta Defensin-2 is a disulfide-linked monomer protein consisting of 42 amino acid residues, and migrates as an approximately 4 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human beta Defensin-2 mature chain was expressed in E. coli.

Human beta Defensin-2

90107-B 20 µg
EUR 205
Description: Recombinant beta Defensin-2 is a disulfide-linked monomer protein consisting of 42 amino acid residues, and migrates as an approximately 4 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human beta Defensin-2 mature chain was expressed in E. coli.

Nori® Human NADPH Oxydase 2 (NOX2) ELISA Kit-2 Plates

GR111143-2 2 x 96-well
EUR 998.4