ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

 

  • This study was conducted to develop a self-assembled direct competitive enzyme-linked immunosorbent assay (dcELISA) kit for the detection of deoxynivalenol (DON) in food and feed grains.

 

    • Based on the preparation of anti-DON monoclonal antibodies, we established a standard curve with dcELISA and optimized the detection conditions.

 

 

    • The performance of the kit was evaluated by comparison with high-performance liquid chromatography (HPLC). The minimum detection limit of DON with the kit was 0.62 ng/mL, the linear range was from 1.0 to 113.24 ng/mL and the half-maximal inhibition concentration (IC50) was 6.61 ng/mL in the working buffer; there was a limit of detection (LOD) of 62 ng/g, and the detection range was from 100 to 11324 ng/g in authentic agricultural samples.

 

    • We examined four samples of wheat bran, wheat flour, corn flour and corn for DON recovery. The average recovery was in the range of 77.1% to 107.0%, and the relative standard deviation (RSD) ranged from 4.2% to 11.9%. In addition, the kit has the advantages of high specificity, good stability, a long effective life and negligible sample matrix interference. Finally, wheat samples from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China were analyzed by the kit.

      iotanano
      iotanano

 

    • A total of 30 samples were randomly checked (five samples in each province), and the results were in good agreement with the standardized HPLC method.

 

  • These tests showed that the dcELISA kit had good performance and met relevant technical requirements, and it had the characteristics of accuracy, reliability, convenience and high-throughput screening for DON detection. Therefore, the developed kit is suitable for rapid screening of DON in marketed products.

 

BD-1 Beta Defensin-1 Mouse Recombinant Protein

PROTP56386 Regular: 20ug
EUR 317
Description: BD 1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 37 amino acids and having a molecular mass of 4.1 KDa.;The BD 1 Mouse is purified by proprietary chromatographic techniques.

BD-1 Beta Defensin -1 Rat Recombinant Protein

PROTO89117 Regular: 20ug
EUR 317
Description: BD-1 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 37 amino acids and having a molecular mass of 4.1kDa.;The BD-1 is purified by proprietary chromatographic techniques.

Recombinant Human BD-1 (36 a.a.) Protein

PROTP60022-1 20ug
EUR 317
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-1 is a 3.9 kDa protein containing 36 amino acid residues.

Human Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit

EI2200-1 96 Well Plate
EUR 477

Human BD-4/beta-Defensin 4 ELISA Kit

RK00186 96 Tests
EUR 521

Human BD-2/beta-Defensin 2 ELISA Kit

RK00193 96 Tests
EUR 521

BD-2, beta-Defensin 2, human

RC220-13 5ug
EUR 104.38

BD-3, beta-Defensin 3, human

RC220-14 5ug
EUR 104.38

BD-4, beta-Defensin 4, human

RC220-15 5ug
EUR 104.38

Human beta-Defensin 1 (BD-1/hBD-1) control/blocking peptide #1

HBD12-P 100 ug
EUR 164

Rabbit Anti-Human beta-Defensin 1 (BD-1/hBD-1) antiserum #1

HBD12-S 100 ul
EUR 457

Mouse Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit

EMI2200-1 96 Well Plate
EUR 477

Nori® Human b-defensin 1 (BD-1) ELISA Kit

GR111105 96-well
EUR 461

Human TGF-beta-1 AssayMax ELISA Kit

ET3102-1 96 Well Plate
EUR 477

Defensin Beta 2 (BD-2) Antibody

20-abx148604
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Defensin Beta 2 (BD-2) Antibody

20-abx007740
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Defensin Beta 2 (BD-2) Antibody

abx412569-01mg 0.1 mg
EUR 704

Human beta-Defensin 3 (BD-3) control/blocking peptide #1

HBD31-P 100 ug
EUR 164

Human Defensin Beta 2 (BD-2) Protein

abx670204-20ug 20 ug
EUR 551

Recombinant Human BD-3 Protein

PROTP81534-1 20ug
EUR 317
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.

Recombinant Human BD-4 Protein

PROTQ8WTQ1-1 20ug
EUR 317
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces.  In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells.  The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.   BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.  In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes.  Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.

Nori® Porcine b-defensin 1 (BD-1) ELISA Kit

GR113087 96-well
EUR 461

Nori® Equine b-defensin 1 (BD-1) ELISA Kit

GR106586 96-well
EUR 461

Nori® Canine b-defensin 1 (BD-1) ELISA Kit

GR115106 96-well
EUR 461

Rabbit Anti-Human beta-Defensin 1 (BD-1/hBD-1), IgG #1, aff pure

HBD12-A 100 ug
EUR 482

BD-3 Beta Defensin-3 Human Recombinant Protein

PROTP81534 Regular: 20ug
EUR 317
Description: Beta Defensin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5161.2 Dalton. ;The BD-3 is purified by proprietary chromatographic techniques.

BD-4 Beta Defensin-4 Human Recombinant Protein

PROTQ8WTQ1 Regular: 20ug
EUR 317
Description: Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa. ;The BD-4 is purified by proprietary chromatographic techniques.

Defensin Beta 2 (BD-2) Antibody (Biotin)

abx412570-50ug 50 ug
EUR 718

Nori® Human b-defensin 1 (BD-1) ELISA Kit- 2 Plates

GR111105-2 2 x 96-well
EUR 832

Human beta-Defensin-2 (BD-2) ELISA Kit, 96 tests, Quantitative

100-250-BD2 1 Kit
EUR 895

Human beta-Defensin-3 (BD-3) ELISA Kit, 96 tests, Quantitative

100-260-BD3 1 kit
EUR 895

Human beta-Defensin 2 (BD-2/hBD-2)control/blocking peptide #1

HBD21-P 100 ug
EUR 164

 

 

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.

Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages.

 

Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F.

 

hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees.

 

Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied.Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds.

 

In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples.

 

Nori® Porcine b-defensin 1 (BD-1) ELISA Kit- 2 Plates

GR113087-2 2 x 96-well
EUR 832

BD-2, beta-Defensin 2, human

RC220-13 5ug
EUR 104.38

Human BD-2/beta-Defensin 2 ELISA Kit

RK00193 96 Tests
EUR 521

Recombinant Human BD-2 Protein

PROTO15263-2 20ug
EUR 317
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-2 is a 4.3 kDa protein containing 41 amino acid residues.

Defensin Beta 2 (BD-2) Antibody

20-abx148604
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Defensin Beta 2 (BD-2) Antibody

20-abx007740
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Defensin Beta 2 (BD-2) Antibody

abx412569-01mg 0.1 mg
EUR 704

Human Defensin Beta 2 (BD-2) Protein

abx670204-20ug 20 ug
EUR 551

Defensin Beta 2 (BD-2) Antibody (Biotin)

abx412570-50ug 50 ug
EUR 718

BD-2 Beta Defensin-2 Human Recombinant Protein

PROTO15263-1 Regular: 20ug
EUR 317
Description: Beta Defensin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.3 kDa. ;The BD-2 is purified by proprietary chromatographic techniques.

Nori® Porcine b-defensin 2 ELISA Kit- 2 Plates

GR113081-2 2 x 96-well
EUR 832

BD-2 Beta Defensin-2 Mouse Recombinant Protein

PROTO15263 Regular: 20ug
EUR 317
Description: Beta Defensin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.5kDa. ;The BD-2 is purified by proprietary chromatographic techniques.

Human beta-Defensin-2 (BD-2) ELISA Kit, 96 tests, Quantitative

100-250-BD2 1 Kit
EUR 895

Nori Human LH beta ELISA Kit-2 Plates

GR111054-2 2 x 96-well
EUR 832

Human beta-Defensin 2 (BD-2/hBD-2)control/blocking peptide #1

HBD21-P 100 ug
EUR 164

Rabbit Anti-Human beta-Defensin 2 (BD-2/hBD-2)antiserum #1

HBD21-S 100 ul
EUR 457

Nori Porcine LH beta ELISA Kit-2 Plates

GR113014-2 2 x 96-well
EUR 832

Nori® Human b-defensin 2 (BD-2) ELISA Kit

GR111099 96-well
EUR 461

Nori® Human NRG1-beta 1/HRG1-beta 1 ELISA Kit (2 plates)

GR106283-2 2 x 96-well
EUR 832

Nori® Human PKA RI-beta ELISA Kit- 2 Plates

GR111083-2 2 x 96-well
EUR 832

Nori® Human TGF beta 1 ELISA Kit-2 Plates

GR106058-2 2 x 96-well
EUR 832

Nori® Human IL-1 beta ELISA Kit (2 plates)

GR106393-2 2 x 96-well
EUR 832

Human BD-4/beta-Defensin 4 ELISA Kit

RK00186 96 Tests
EUR 521

Human BD-1/beta-Defensin 1 ELISA Kit

RK00211 96 Tests
EUR 521

BD-3, beta-Defensin 3, human

RC220-14 5ug
EUR 104.38

BD-4, beta-Defensin 4, human

RC220-15 5ug
EUR 104.38

Recombinant Human BD-1 (47a.a.) Protein

PROTP60022-2 20ug
EUR 317
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

Nori Human TIM-2 ELISA Kit-2 Plates

GR111039-2 2 x 96-well
EUR 832

Nori® Human Angiopoietin-2 (ANG-2) ELISA Kit (2 plates)

GR106245-2 2 x 96-well
EUR 832

Nori® Bovine PKA RI-beta ELISA Kit- 2 Plates

GR112036-2 2 x 96-well
EUR 832

Nori® Porcine PKA RI-beta ELISA Kit- 2 Plates

GR113069-2 2 x 96-well
EUR 832

Nori® Equine PKA RI-beta ELISA Kit- 2 Plates

GR106567-2 2 x 96-well
EUR 832

Nori® Chicken PKA RI-beta ELISA Kit- 2 Plates

GR114016-2 2 x 96-well
EUR 832

Nori® Human Tie-2 ELISA Kit (2 plates)

GR106458-2 2 x 96-well
EUR 832

Nori® Human TIMP-2 ELISA Kit (2 plates)

GR106460-2 2 x 96-well
EUR 832

Nori® Human Trappin-2 ELISA Kit (2 plates)

GR106467-2 2 x 96-well
EUR 832

Nori® Human MMP-2/TIMP-2 Complex ELISA Kit (2 plates)

GR106372-2 2 x 96-well
EUR 832

Recombinant Human TGF-Beta 2 (insect derived) Protein

PROTP61812-2 10ug
EUR 317
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-β2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
* Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

Human beta defensin 2 ELISA Kit

ELA-E0377h 96 Tests
EUR 824

Anti-beta 2 Microglobulin/B2m Antibody

A00456-2 100ug/vial
EUR 294

Nori® Equine (horse) TGF-beta 1 ELISA Kit (2 plates)

GR106123-2 2 x 96-well
EUR 832

Nori® Bovine (cattle) TGF-beta 1 ELISA Kit (2 plates)

GR106124-2 2 x 96-well
EUR 832

Nori® Canine (dog) TGF-beta 1 ELISA Kit (2 plates)

GR106125-2 2 x 96-well
EUR 832

Nori® Porcine (pig) TGF-beta 1 ELISA Kit (2 plates)

GR106126-2 2 x 96-well
EUR 832

BD-1, beta-Defensin 1 (47a.a), human

RC220-12 5ug
EUR 104.38

Rabbit Anti-Human beta-Defensin 2 (BD-2/hBD-2), IgG #1, aff pure

HBD21-A 100 ug
EUR 482

Nori Equine MMP-2 ELISA Kit-2 Plates

GR106478-2 2 x 96-well
EUR 832

Nori Equine TIM-2 ELISA Kit (2 plates)

GR106516-2 2 x 96-well
EUR 832

Nori Canine TIM-2 ELISA Kit (2 plates)

GR115041-2 2 x 96-well
EUR 832

Nori Equine MMP-2/TIMP-2 Complex ELISA Kit (2 plates)

GR106502-2 2 x 96-well
EUR 832

B2M Human, Beta 2 Microglobulin Human Recombinant Protein, His Tag

PROTP61769-2 Regular: 5ug
EUR 317
Description: B2M Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids (1-119 a.a.) and having a molecular mass of 14 kDa. The B2M is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Nori® Human NADPH Oxydase 2 (NOX2) ELISA Kit-2 Plates

GR111143-2 2 x 96-well
EUR 832

Anti-Beta 2 Microglobulin Antibody (monoclonal, 2H10)

M00456-2 100ug/vial
EUR 334

Nori Human A1AT ELISA Kit-2 Plates

GR111022-2 2 x 96-well
EUR 832

Nori Human A1PI ELISA Kit-2 Plates

GR111023-2 2 x 96-well
EUR 832

Nori Human LDHA ELISA Kit-2 Plates

GR111024-2 2 x 96-well
EUR 832

Nori Human CRP ELISA Kit-2 Plates

GR111025-2 2 x 96-well
EUR 832

Nori Human ACE ELISA Kit-2 Plates

GR111026-2 2 x 96-well
EUR 832

Nori Human Uteroglobin ELISA Kit-2 Plates

GR111027-2 2 x 96-well
EUR 832

Nori Human eNOS ELISA Kit-2 Plates

GR111031-2 2 x 96-well
EUR 832

Nori Human PCNA ELISA Kit-2 Plates

GR111032-2 2 x 96-well
EUR 832

Nori Human DR3 ELISA Kit-2 Plates

GR111049-2 2 x 96-well
EUR 832