ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat.

Development of a Direct Competitive ELISA Kit for Detecting Deoxynivalenol Contamination in Wheat

 

  • This study was conducted to develop a self-assembled direct competitive enzyme-linked immunosorbent assay (dcELISA) kit for the detection of deoxynivalenol (DON) in food and feed grains.

 

    • Based on the preparation of anti-DON monoclonal antibodies, we established a standard curve with dcELISA and optimized the detection conditions.

 

 

    • The performance of the kit was evaluated by comparison with high-performance liquid chromatography (HPLC). The minimum detection limit of DON with the kit was 0.62 ng/mL, the linear range was from 1.0 to 113.24 ng/mL and the half-maximal inhibition concentration (IC50) was 6.61 ng/mL in the working buffer; there was a limit of detection (LOD) of 62 ng/g, and the detection range was from 100 to 11324 ng/g in authentic agricultural samples.

 

    • We examined four samples of wheat bran, wheat flour, corn flour and corn for DON recovery. The average recovery was in the range of 77.1% to 107.0%, and the relative standard deviation (RSD) ranged from 4.2% to 11.9%. In addition, the kit has the advantages of high specificity, good stability, a long effective life and negligible sample matrix interference. Finally, wheat samples from farms in the six provinces of Henan, Anhui, Hebei, Shandong, Jiangsu and Gansu in China were analyzed by the kit.

      iotanano
      iotanano

 

    • A total of 30 samples were randomly checked (five samples in each province), and the results were in good agreement with the standardized HPLC method.

 

  • These tests showed that the dcELISA kit had good performance and met relevant technical requirements, and it had the characteristics of accuracy, reliability, convenience and high-throughput screening for DON detection. Therefore, the developed kit is suitable for rapid screening of DON in marketed products.

 

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
ELISA-1 1
EUR 202.00
BD-1 Beta Defensin-1 Human Recombinant Protein
PROTP60022 Regular: 20ug
EUR 317.00
Description: Beta Defensin-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.;The BD-1 is purified by proprietary chromatographic techniques.
Human beta-Defensin-1 (BD-1) ELISA Kit, 96 tests, Quantitative
100-240-BD1 1 kit
EUR 895.00
BD-1 Beta Defensin-1 Mouse Recombinant Protein
PROTP56386 Regular: 20ug
EUR 317.00
Description: BD 1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 37 amino acids and having a molecular mass of 4.1 KDa.;The BD 1 Mouse is purified by proprietary chromatographic techniques.
BD-1 Beta Defensin -1 Rat Recombinant Protein
PROTO89117 Regular: 20ug
EUR 317.00
Description: BD-1 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 37 amino acids and having a molecular mass of 4.1kDa.;The BD-1 is purified by proprietary chromatographic techniques.
Recombinant Human BD-1 (36 a.a.) Protein
PROTP60022-1 20ug
EUR 317.00
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-1 is a 3.9 kDa protein containing 36 amino acid residues.
Human BD-4/beta-Defensin 4 ELISA Kit
RK00186 96 Tests
EUR 521.00
Human BD-2/beta-Defensin 2 ELISA Kit
RK00193 96 Tests
EUR 521.00
Human Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit
EI2200-1 96 Well Plate
EUR 477.00
BD-2, beta-Defensin 2, human
RC220-13 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Defensins
BD-3, beta-Defensin 3, human
RC220-14 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Defensins
BD-4, beta-Defensin 4, human
RC220-15 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Defensins
Human beta-Defensin 1 (BD-1/hBD-1) control/blocking peptide #1
HBD12-P 100 ug
EUR 164.00
Rabbit Anti-Human beta-Defensin 1 (BD-1/hBD-1) antiserum #1
HBD12-S 100 ul
EUR 457.00
Mouse Interleukin-1 beta (IL-1 beta) AssayMax ELISA Kit
EMI2200-1 96 Well Plate
EUR 477.00
Human TGF-beta-1 AssayMax ELISA Kit
ET3102-1 96 Well Plate
EUR 477.00
Defensin Beta 2 (BD-2) Antibody
20-abx148604
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Defensin Beta 2 (BD-2) Antibody
20-abx007740
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Defensin Beta 2 (BD-2) Antibody
abx412569-01mg 0.1 mg
EUR 704.00
  • Shipped within 1 week.
Human Defensin Beta 2 (BD-2) Protein
abx670204-20ug 20 ug
EUR 551.00
  • Shipped within 1 week.
Human beta-Defensin 3 (BD-3) control/blocking peptide #1
HBD31-P 100 ug
EUR 164.00
Recombinant Human BD-3 Protein
PROTP81534-1 20ug
EUR 317.00
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.
Recombinant Human BD-4 Protein
PROTQ8WTQ1-1 20ug
EUR 317.00
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces.  In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells.  The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.   BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.  In addition to its direct antimicrobial activities, BD-4 is chemoattractant towards human blood monocytes.  Recombinant human BD-4 is a 6.0 kDa protein containing 50 amino acid residues.
Rabbit Anti-Human beta-Defensin 1 (BD-1/hBD-1), IgG #1, aff pure
HBD12-A 100 ug
EUR 482.00
BD-3 Beta Defensin-3 Human Recombinant Protein
PROTP81534 Regular: 20ug
EUR 317.00
Description: Beta Defensin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5161.2 Dalton. ;The BD-3 is purified by proprietary chromatographic techniques.
BD-4 Beta Defensin-4 Human Recombinant Protein
PROTQ8WTQ1 Regular: 20ug
EUR 317.00
Description: Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa. ;The BD-4 is purified by proprietary chromatographic techniques.
Defensin Beta 2 (BD-2) Antibody (Biotin)
abx412570-50ug 50 ug
EUR 718.00
  • Shipped within 1 week.
Human beta-Defensin-2 (BD-2) ELISA Kit, 96 tests, Quantitative
100-250-BD2 1 Kit
EUR 895.00
Human beta-Defensin-3 (BD-3) ELISA Kit, 96 tests, Quantitative
100-260-BD3 1 kit
EUR 895.00
Human beta-Defensin 2 (BD-2/hBD-2)control/blocking peptide #1
HBD21-P 100 ug
EUR 164.00
Rabbit Anti-Human beta-Defensin 2 (BD-2/hBD-2)antiserum #1
HBD21-S 100 ul
EUR 457.00
Rabbit Anti-human beta-Defensin 3 (BD-3) IgG #1 aff pure
HBD31-A 100 ug
EUR 482.00

 

 

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.

Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages.

 

Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F.

 

hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees.

 

Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied.Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds.

 

In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples.

 

Recombinant Human BD-2 Protein

PROTO15263-2 20ug
EUR 317.00
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-2 is a 4.3 kDa protein containing 41 amino acid residues.

Defensin Beta 2 (BD-2) Antibody

20-abx007740
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Defensin Beta 2 (BD-2) Antibody

20-abx148604
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Defensin Beta 2 (BD-2) Antibody

abx412569-01mg 0.1 mg
EUR 704.00
  • Shipped within 1 week.

Human Defensin Beta 2 (BD-2) Protein

abx670204-20ug 20 ug
EUR 551.00
  • Shipped within 1 week.

Defensin Beta 2 (BD-2) Antibody (Biotin)

abx412570-50ug 50 ug
EUR 718.00
  • Shipped within 1 week.

BD-2 Beta Defensin-2 Human Recombinant Protein

PROTO15263-1 Regular: 20ug
EUR 317.00
Description: Beta Defensin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.3 kDa. ;The BD-2 is purified by proprietary chromatographic techniques.

Human beta-Defensin-2 (BD-2) ELISA Kit, 96 tests, Quantitative

100-250-BD2 1 Kit
EUR 895.00

BD-2 Beta Defensin-2 Mouse Recombinant Protein

PROTO15263 Regular: 20ug
EUR 317.00
Description: Beta Defensin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.5kDa. ;The BD-2 is purified by proprietary chromatographic techniques.

Human BD-4/beta-Defensin 4 ELISA Kit

RK00186 96 Tests
EUR 521.00

Human BD-1/beta-Defensin 1 ELISA Kit

RK00211 96 Tests
EUR 521.00

Recombinant Human BD-1 (47a.a.) Protein

PROTP60022-2 20ug
EUR 317.00
Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human BD-1 is a 5.0 kDa protein containing 47 amino acid residues.

Human beta-Defensin 2 (BD-2/hBD-2)control/blocking peptide #1

HBD21-P 100 ug
EUR 164.00

Rabbit Anti-Human beta-Defensin 2 (BD-2/hBD-2)antiserum #1

HBD21-S 100 ul
EUR 457.00

BD-3, beta-Defensin 3, human

RC220-14 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Defensins

BD-4, beta-Defensin 4, human

RC220-15 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Defensins

Recombinant Human TGF-Beta 2 (insect derived) Protein

PROTP61812-2 10ug
EUR 317.00
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-β2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
* Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

BD-1, beta-Defensin 1 (47a.a), human

RC220-12 5ug
EUR 104.38
  • Product category: Proteins/Recombinant Proteins/Defensins

Anti-beta 2 Microglobulin/B2m Antibody

A00456-2 100ug/vial
EUR 294.00

Rabbit Anti-Human beta-Defensin 2 (BD-2/hBD-2), IgG #1, aff pure

HBD21-A 100 ug
EUR 482.00

Human beta defensin 2 ELISA Kit

ELA-E0377h 96 Tests
EUR 824.00

B2M Human, Beta 2 Microglobulin Human Recombinant Protein, His Tag

PROTP61769-2 Regular: 5ug
EUR 317.00
Description: B2M Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids (1-119 a.a.) and having a molecular mass of 14 kDa. The B2M is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Anti-Beta 2 Microglobulin Antibody (monoclonal, 2H10)

M00456-2 100ug/vial
EUR 334.00

BD-1 Beta Defensin-1 Human Recombinant Protein

PROTP60022 Regular: 20ug
EUR 317.00
Description: Beta Defensin-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.;The BD-1 is purified by proprietary chromatographic techniques.

BD-3 Beta Defensin-3 Human Recombinant Protein

PROTP81534 Regular: 20ug
EUR 317.00
Description: Beta Defensin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5161.2 Dalton. ;The BD-3 is purified by proprietary chromatographic techniques.

BD-4 Beta Defensin-4 Human Recombinant Protein

PROTQ8WTQ1 Regular: 20ug
EUR 317.00
Description: Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa. ;The BD-4 is purified by proprietary chromatographic techniques.

BD-2, Human

HY-P7135 50ug
EUR 533.00

Human Defensin Beta 2 (DEFB2) ELISA Kit

abx051085-96tests 96 tests
EUR 707.00
  • Shipped within 5-12 working days.

Human Defensin Beta 2 (DEFB2) ELISA Kit

20-abx151285
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Defensin Beta 2 (DEFB2) ELISA Kit

abx354352-96tests 96 tests
EUR 707.00
  • Shipped within 5-12 working days.

Human Defensin Beta 2 (DEFb2) ELISA Kit

DLR-DEFb2-Hu-48T 48T
EUR 479.00
  • Should the Human Defensin Beta 2 (DEFb2) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Defensin Beta 2 (DEFb2) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Defensin Beta 2 (DEFb2) ELISA Kit

DLR-DEFb2-Hu-96T 96T
EUR 621.00
  • Should the Human Defensin Beta 2 (DEFb2) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Defensin Beta 2 (DEFb2) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Defensin Beta 2 (DEFb2) ELISA Kit

DL-DEFb2-Hu-192 1 kit of 192 tests
EUR 1054.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Human Defensin Beta 2 (DEFb2)

Human Defensin Beta 2 (DEFb2) ELISA Kit

DL-DEFb2-Hu-48 1 kit of 48 tests
EUR 447.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Human Defensin Beta 2 (DEFb2)

Human Defensin Beta 2 (DEFb2) ELISA Kit

DL-DEFb2-Hu-96 1 kit of 96 tests
EUR 597.00
Description: An ELISA kit based on the sandwich method for detection and quantification of Human Defensin Beta 2 (DEFb2)

Human Defensin Beta 2 (DEFb2) ELISA Kit

RDR-DEFb2-Hu-48Tests 48 Tests
EUR 500.00

Human Defensin Beta 2 (DEFb2) ELISA Kit

RDR-DEFb2-Hu-96Tests 96 Tests
EUR 692.00

Human Defensin Beta 2 ELISA Kit (DEFb2)

RK01264 96 Tests
EUR 521.00

Human Defensin Beta 2 (DEFb2) ELISA Kit

RD-DEFb2-Hu-48Tests 48 Tests
EUR 478.00

Human Defensin Beta 2 (DEFb2) ELISA Kit

RD-DEFb2-Hu-96Tests 96 Tests
EUR 662.00

Human Defensin Beta 2 (DEFb2) ELISA Kit

SEA072Hu-10x96wellstestplate 10x96-wells test plate
EUR 4273.35
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Defensin Beta 2 (DEFb2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Defensin Beta 2 (DEFb2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Defensin Beta 2 (DEFb2) ELISA Kit

SEA072Hu-1x48wellstestplate 1x48-wells test plate
EUR 439.57
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Defensin Beta 2 (DEFb2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Defensin Beta 2 (DEFb2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Defensin Beta 2 (DEFb2) ELISA Kit

SEA072Hu-1x96wellstestplate 1x96-wells test plate
EUR 585.10
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Defensin Beta 2 (DEFb2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Defensin Beta 2 (DEFb2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Defensin Beta 2 (DEFb2) ELISA Kit

SEA072Hu-5x96wellstestplate 5x96-wells test plate
EUR 2332.95
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Defensin Beta 2 (DEFb2) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Defensin Beta 2 (DEFb2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Defensin Beta 2 (DEFb2) ELISA Kit

4-SEA072Hu
  • EUR 4324.00
  • EUR 2283.00
  • EUR 586.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Defensin Beta 2 elisa. Alternative names of the recognized antigen: B-DF2
  • DEFB2
  • BD2
  • DEF-B2
  • HBD2
  • DEFB102
  • HBD-2
  • SAP1
  • DEFB4A
  • DEFB4
  • Skin-Antimicrobial Peptide 1
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Defensin Beta 2 (DEFb2) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse beta defensin 2 ELISA Kit

ELA-E0377m 96 Tests
EUR 865.00

Human beta-Defensin-1 (BD-1) ELISA Kit, 96 tests, Quantitative

100-240-BD1 1 kit
EUR 895.00

Human beta-Defensin-3 (BD-3) ELISA Kit, 96 tests, Quantitative

100-260-BD3 1 kit
EUR 895.00

Human DEFβ2/DEFB2(Defensin Beta 2) ELISA Kit

E-EL-H0996-192 192 tests
EUR 895.00
  • No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.
Description: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFβ2/DEFB2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the OD of the samples to the standard curve.

Human DEFβ2/DEFB2(Defensin Beta 2) ELISA Kit

E-EL-H0996-96 96 tests
EUR 530.00
  • No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.
Description: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFβ2/DEFB2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the OD of the samples to the standard curve.

Recombinant Human Beta Defensin -2

CC017-005 5ug
EUR 293.00

Recombinant Human Beta Defensin -2

CC017-020 20ug
EUR 408.00

Recombinant Human Beta Defensin -2

CC017-101 1mg
EUR 5018.00

Recombinant Human Beta Defensin -2

7-00124 5µg Ask for price

Recombinant Human Beta Defensin -2

7-00125 20µg Ask for price

Recombinant Human Beta Defensin -2

7-00126 1mg Ask for price

BD-4 Beta Defensin-4 Rat Recombinant Protein

PROTO88514 Regular: 20ug
EUR 317.00
Description: BD-4 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.4kDa.;The BD-4 is purified by proprietary chromatographic techniques.

BD-1 Beta Defensin -1 Rat Recombinant Protein

PROTO89117 Regular: 20ug
EUR 317.00
Description: BD-1 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 37 amino acids and having a molecular mass of 4.1kDa.;The BD-1 is purified by proprietary chromatographic techniques.

BD-1 Beta Defensin-1 Mouse Recombinant Protein

PROTP56386 Regular: 20ug
EUR 317.00
Description: BD 1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 37 amino acids and having a molecular mass of 4.1 KDa.;The BD 1 Mouse is purified by proprietary chromatographic techniques.

BD-3 Beta Defensin-3 Rat Recombinant Protein

PROTQ32ZI4 Regular: 20ug
EUR 317.00
Description: BD-3 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.5kDa.;The BD-3 is purified by proprietary chromatographic techniques.

BD-3 Beta Defensin-3 Mouse Recombinant Protein

PROTQ9WTL0 Regular: 20ug
EUR 317.00
Description: Beta Defensin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.6kDa. ;The BD-3 is purified by proprietary chromatographic techniques.

IL-2 Interleukin-2 Human Recombinant Protein, His Tag

PROTP60568-2 Regular: 10ug
EUR 317.00
Description: Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 133 amino acids fragment (21-153) having a molecular weight of 20kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. _x000D_ The IL-2 His is purified by proprietary chromatographic techniques._x000D_