As a result of issues raised by way of animal or microbial recombinant proteases, using proteases from vegetable origin is changing into more and more in style. Amongst them, sulfidryl proteases have a particular curiosity. Fiction is an excellent instance of this type of protease.
This paper goals to be to make a complete evaluation of the current makes use of of this enzyme, together with for instance protein hydrolysis, the manufacturing of bioactive peptides and antibodies fragments (researchers level that ficin outcomes are extra reproducible than utilizing different proteases), meat tenderization, milk coagulations in cheese making or peptide synthesis.
Efforts to get industrial immobilized biocatalysts of the enzyme will likely be additionally described. The evaluation reveals the huge potential and sensible prospect that this enzyme can have within the close to future.
An alternate for proteinase Ok-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and particular utility for protein sequencing
Description: Quantitative sandwich ELISA kit for measuring Mouse adiponectin, ADP in samples from serum, plasma, cell culture supernates, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative sandwich ELISA kit for measuring Mouse adiponectin, ADP in samples from serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Adiponectin (ADP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Description of target: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 10 pg/ml
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Total Adiponectin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse adiponectin,ADP in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Adiponectin (ADP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Background: A impartial, heat-sensitive serine protease (NHSSP) originating from the feather-degrading fungus Onygena corvina (O. corvina) was described and outlined as an alkaline serine protease of the subtilisin kind S8 household, exhibiting an enzymatic exercise at impartial pH. Usually, broad specificity proteases, akin to proteinase Ok or trypsin, have discovered quite a few purposes in analysis and biotechnology.
Outcomes: We report the cloning and expression within the yeast PichiaPink™ system, in addition to purification, and characterization of the NHSSP. Recombinant, His6-tagged NHSSP was effectively expressed from an optimized, artificial gene and purified utilizing a easy protocol based mostly on ammonium sulfate fractionation and hydrophobic interplay chromatography.
The enzyme reveals atypical C-terminal processing, the coded preproprotein undergoes sign peptide removing and maturation by means of the clipping of a propeptide part and 10 amino acids (aa) from the C-terminus, together with the His6-tag.
The deletion variant has been constructed, devoid of the C-terminal ORF section, thus eliminating the necessity for C-terminal processing. Each NHSSP variants exhibit very related enzymatic traits.
The purified enzymes have been characterised to find out the optimum proteolytic circumstances. We revealed that the mature NHSSP is reproducibly lively over a large pH vary from impartial to delicate acidic (pH of 5.zero to eight.5), with an optimum at pH 6.8, and at temperatures of 15 to 50 °C with an optimum at 38-42 °C.
Apparently, we demonstrated that the protease could be totally deactivated by a average enhance in temperature of about 15 °C from the optimum to over 50 °C.
The protease was partially delicate to serine protease inhibitors, and never inhibited by chelating or decreasing brokers and detergents. SDS induced autolysis of NHSSP, which factors to a excessive stimulation of its proteolytic exercise.
Conclusions: The NHSSP was produced as a recombinant protein with excessive effectivity. In comparison with proteinase Ok, the commonest serine protease used, NHSSP reveals an approx. twofold increased particular exercise.
Protein sequencing is usually a beneficial technical utility for the protease. The protein protection is considerably increased compared to trypsin and reaches about 84-100% for β-lactoglobulin (BLG), antibody (mAb) mild and heavy chains.
Moreover, the choice to carry out digestions at impartial to barely acidic pH-values right down to pH 5.zero avoids modification of peptides, e.g. because of deamidation.
Key phrases: Expression; Meals processing; Onygena corvina; Pichia pastoris; Protease; Protein degradation; Protein sequencing; Proteinase Ok; Serine protease; Waste processing.
Description: Quantitative competitive ELISA kit for measuring Mouse Albumin (Alb) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Mouse Albumin (Alb) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Albumin (ALB) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A competitive ELISA for quantitative measurement of Mouse Glycated Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Glycated Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Glycated Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Pre Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Pre Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Pre Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Description of target: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.704 ng/ml
Description: A sandwich ELISA for quantitative measurement of Mouse Ischemia Modified Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Ischemia Modified Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Ischemia Modified Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Ischemia Modified Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Ischemia Modified Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Ischemia Modified Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive Inhibition ELISA kit for detection of Albumin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA for quantitative measurement of Mouse Anti Albumin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Anti Albumin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Anti Albumin Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Bioactive molecules from protists: Views in biotechnology
For a whole bunch of years, mankind has benefited from the pure metabolic processes of microorganisms to acquire primary merchandise akin to fermented meals and alcoholic drinks.
Extra lately, microorganisms have been exploited for the manufacturing of antibiotics, nutritional vitamins and enzymes for use in medication and chemical industries.
Moreover, a number of fashionable medication, together with these for most cancers remedy, are pure merchandise or their derivatives.
Protists are a nonetheless underexplored supply of pure merchandise probably of curiosity for biotechnological and biomedical purposes.
This paper focuses on some examples of bioactive molecules from protists and related micro organism and their potential use in biotechnology.
Key phrases: Bioactive molecules from protists; Climacostol; Chilly-adapted enzymes; Ice binding proteins; Pure merchandise; Secondary metabolites.
